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A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Optical Society of America
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378349/ https://www.ncbi.nlm.nih.gov/pubmed/20389554 http://dx.doi.org/10.1364/OE.18.005390 |