A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection

We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam...

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Detalles Bibliográficos
Autores principales: McMullen, Jesse D., Zipfel, Warren R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Research-Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378349/
https://www.ncbi.nlm.nih.gov/pubmed/20389554
http://dx.doi.org/10.1364/OE.18.005390
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author McMullen, Jesse D.
Zipfel, Warren R.
author_facet McMullen, Jesse D.
Zipfel, Warren R.
author_sort McMullen, Jesse D.
collection PubMed
description We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs.
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spelling pubmed-33783492012-10-01 A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection McMullen, Jesse D. Zipfel, Warren R. Opt Express Research-Article We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs. Optical Society of America 2010-03-01 /pmc/articles/PMC3378349/ /pubmed/20389554 http://dx.doi.org/10.1364/OE.18.005390 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
McMullen, Jesse D.
Zipfel, Warren R.
A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
title A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
title_full A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
title_fullStr A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
title_full_unstemmed A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
title_short A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
title_sort multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378349/
https://www.ncbi.nlm.nih.gov/pubmed/20389554
http://dx.doi.org/10.1364/OE.18.005390
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