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Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169
Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family expressed on macrophages. Its macrophage specific expression makes it an attractive target for delivering antigens to tissue macrophages via Sn-mediated endocytosis. Here we describe a novel appr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378521/ https://www.ncbi.nlm.nih.gov/pubmed/22723922 http://dx.doi.org/10.1371/journal.pone.0039039 |
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author | Chen, Weihsu C. Kawasaki, Norihito Nycholat, Corwin M. Han, Shoufa Pilotte, Julie Crocker, Paul R. Paulson, James C. |
author_facet | Chen, Weihsu C. Kawasaki, Norihito Nycholat, Corwin M. Han, Shoufa Pilotte, Julie Crocker, Paul R. Paulson, James C. |
author_sort | Chen, Weihsu C. |
collection | PubMed |
description | Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family expressed on macrophages. Its macrophage specific expression makes it an attractive target for delivering antigens to tissue macrophages via Sn-mediated endocytosis. Here we describe a novel approach for delivering antigens to macrophages using liposomal nanoparticles displaying high affinity glycan ligands of Sn. The Sn-targeted liposomes selectively bind to and are internalized by Sn-expressing cells, and accumulate intracellularly over time. Our results show that ligand decorated liposomes are specific for Sn, since they are taken up by bone marrow derived macrophages that are derived from wild type but not Sn(−/−) mice. Importantly, the Sn-targeted liposomes dramatically enhance the delivery of antigens to macrophages for presentation to and proliferation of antigen-specific T cells. Together, these data provide insights into the potential of cell-specific targeting and delivery of antigens to intracellular organelles of macrophages using Sn-ligand decorated liposomal nanoparticles. |
format | Online Article Text |
id | pubmed-3378521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33785212012-06-21 Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 Chen, Weihsu C. Kawasaki, Norihito Nycholat, Corwin M. Han, Shoufa Pilotte, Julie Crocker, Paul R. Paulson, James C. PLoS One Research Article Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family expressed on macrophages. Its macrophage specific expression makes it an attractive target for delivering antigens to tissue macrophages via Sn-mediated endocytosis. Here we describe a novel approach for delivering antigens to macrophages using liposomal nanoparticles displaying high affinity glycan ligands of Sn. The Sn-targeted liposomes selectively bind to and are internalized by Sn-expressing cells, and accumulate intracellularly over time. Our results show that ligand decorated liposomes are specific for Sn, since they are taken up by bone marrow derived macrophages that are derived from wild type but not Sn(−/−) mice. Importantly, the Sn-targeted liposomes dramatically enhance the delivery of antigens to macrophages for presentation to and proliferation of antigen-specific T cells. Together, these data provide insights into the potential of cell-specific targeting and delivery of antigens to intracellular organelles of macrophages using Sn-ligand decorated liposomal nanoparticles. Public Library of Science 2012-06-19 /pmc/articles/PMC3378521/ /pubmed/22723922 http://dx.doi.org/10.1371/journal.pone.0039039 Text en Chen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Weihsu C. Kawasaki, Norihito Nycholat, Corwin M. Han, Shoufa Pilotte, Julie Crocker, Paul R. Paulson, James C. Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 |
title | Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 |
title_full | Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 |
title_fullStr | Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 |
title_full_unstemmed | Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 |
title_short | Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 |
title_sort | antigen delivery to macrophages using liposomal nanoparticles targeting sialoadhesin/cd169 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378521/ https://www.ncbi.nlm.nih.gov/pubmed/22723922 http://dx.doi.org/10.1371/journal.pone.0039039 |
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