Cargando…
Polyadenylation helps regulate functional tRNA levels in Escherichia coli
Here we demonstrate a new regulatory mechanism for tRNA processing in Escherichia coli whereby RNase T and RNase PH, the two primary 3′ → 5′ exonucleases involved in the final step of 3′-end maturation, compete with poly(A) polymerase I (PAP I) for tRNA precursors in wild-type cells. In the absence...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378859/ https://www.ncbi.nlm.nih.gov/pubmed/22287637 http://dx.doi.org/10.1093/nar/gks006 |
_version_ | 1782236090692796416 |
---|---|
author | Mohanty, Bijoy K. Maples, Valerie F. Kushner, Sidney R. |
author_facet | Mohanty, Bijoy K. Maples, Valerie F. Kushner, Sidney R. |
author_sort | Mohanty, Bijoy K. |
collection | PubMed |
description | Here we demonstrate a new regulatory mechanism for tRNA processing in Escherichia coli whereby RNase T and RNase PH, the two primary 3′ → 5′ exonucleases involved in the final step of 3′-end maturation, compete with poly(A) polymerase I (PAP I) for tRNA precursors in wild-type cells. In the absence of both RNase T and RNase PH, there is a >30-fold increase of PAP I-dependent poly(A) tails that are ≤10 nt in length coupled with a 2.3- to 4.2-fold decrease in the level of aminoacylated tRNAs and a >2-fold decrease in growth rate. Only 7 out of 86 tRNAs are not regulated by this mechanism and are also not substrates for RNase T, RNase PH or PAP I. Surprisingly, neither PNPase nor RNase II has any effect on tRNA poly(A) tail length. Our data suggest that the polyadenylation of tRNAs by PAP I likely proceeds in a distributive fashion unlike what is observed with mRNAs. |
format | Online Article Text |
id | pubmed-3378859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33788592012-06-20 Polyadenylation helps regulate functional tRNA levels in Escherichia coli Mohanty, Bijoy K. Maples, Valerie F. Kushner, Sidney R. Nucleic Acids Res Nucleic Acid Enzymes Here we demonstrate a new regulatory mechanism for tRNA processing in Escherichia coli whereby RNase T and RNase PH, the two primary 3′ → 5′ exonucleases involved in the final step of 3′-end maturation, compete with poly(A) polymerase I (PAP I) for tRNA precursors in wild-type cells. In the absence of both RNase T and RNase PH, there is a >30-fold increase of PAP I-dependent poly(A) tails that are ≤10 nt in length coupled with a 2.3- to 4.2-fold decrease in the level of aminoacylated tRNAs and a >2-fold decrease in growth rate. Only 7 out of 86 tRNAs are not regulated by this mechanism and are also not substrates for RNase T, RNase PH or PAP I. Surprisingly, neither PNPase nor RNase II has any effect on tRNA poly(A) tail length. Our data suggest that the polyadenylation of tRNAs by PAP I likely proceeds in a distributive fashion unlike what is observed with mRNAs. Oxford University Press 2012-05 2012-01-28 /pmc/articles/PMC3378859/ /pubmed/22287637 http://dx.doi.org/10.1093/nar/gks006 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Nucleic Acid Enzymes Mohanty, Bijoy K. Maples, Valerie F. Kushner, Sidney R. Polyadenylation helps regulate functional tRNA levels in Escherichia coli |
title | Polyadenylation helps regulate functional tRNA levels in Escherichia coli |
title_full | Polyadenylation helps regulate functional tRNA levels in Escherichia coli |
title_fullStr | Polyadenylation helps regulate functional tRNA levels in Escherichia coli |
title_full_unstemmed | Polyadenylation helps regulate functional tRNA levels in Escherichia coli |
title_short | Polyadenylation helps regulate functional tRNA levels in Escherichia coli |
title_sort | polyadenylation helps regulate functional trna levels in escherichia coli |
topic | Nucleic Acid Enzymes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378859/ https://www.ncbi.nlm.nih.gov/pubmed/22287637 http://dx.doi.org/10.1093/nar/gks006 |
work_keys_str_mv | AT mohantybijoyk polyadenylationhelpsregulatefunctionaltrnalevelsinescherichiacoli AT maplesvalerief polyadenylationhelpsregulatefunctionaltrnalevelsinescherichiacoli AT kushnersidneyr polyadenylationhelpsregulatefunctionaltrnalevelsinescherichiacoli |