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A robust PCR primer design platform applied to the detection of Acidobacteria Group 1 in soil

Environmental biosurveillance and microbial ecology studies use PCR-based assays to detect and quantify microbial taxa and gene sequences within a complex background of microorganisms. However, the fragmentary nature and growing quantity of DNA-sequence data make group-specific assay design challeng...

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Detalles Bibliográficos
Autores principales: Gans, Jason D., Dunbar, John, Eichorst, Stephanie A., Gallegos-Graves, La Verne, Wolinsky, Murray, Kuske, Cheryl R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384349/
https://www.ncbi.nlm.nih.gov/pubmed/22434885
http://dx.doi.org/10.1093/nar/gks238