Meiotic cohesin complexes are essential for the formation of the axial element in mice

Cohesin is a conserved multisubunit protein complex that participates in chromosome segregation, DNA damage repair, chromatin regulation, and synaptonemal complex (SC) formation. Yeast, but not mice, depleted of the cohesin subunit Rec8 are defective in the formation of the axial elements (AEs) of t...

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Autores principales: Llano, Elena, Herrán, Yurema, García-Tuñón, Ignacio, Gutiérrez-Caballero, Cristina, de Álava, Enrique, Barbero, José Luis, Schimenti, John, de Rooij, Dirk G., Sánchez-Martín, Manuel, Pendás, Alberto M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384418/
https://www.ncbi.nlm.nih.gov/pubmed/22711701
http://dx.doi.org/10.1083/jcb.201201100
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author Llano, Elena
Herrán, Yurema
García-Tuñón, Ignacio
Gutiérrez-Caballero, Cristina
de Álava, Enrique
Barbero, José Luis
Schimenti, John
de Rooij, Dirk G.
Sánchez-Martín, Manuel
Pendás, Alberto M.
author_facet Llano, Elena
Herrán, Yurema
García-Tuñón, Ignacio
Gutiérrez-Caballero, Cristina
de Álava, Enrique
Barbero, José Luis
Schimenti, John
de Rooij, Dirk G.
Sánchez-Martín, Manuel
Pendás, Alberto M.
author_sort Llano, Elena
collection PubMed
description Cohesin is a conserved multisubunit protein complex that participates in chromosome segregation, DNA damage repair, chromatin regulation, and synaptonemal complex (SC) formation. Yeast, but not mice, depleted of the cohesin subunit Rec8 are defective in the formation of the axial elements (AEs) of the SC, suggesting that, in mammals, this function is not conserved. In this paper, we show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51- but not DMC1-mediated double-strand break repair, were not able to assemble their AEs, and arrested as early as the leptotene stage of prophase I, demonstrating that cohesin plays an essential role in AE assembly that is conserved from yeast to mammals.
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spelling pubmed-33844182012-12-25 Meiotic cohesin complexes are essential for the formation of the axial element in mice Llano, Elena Herrán, Yurema García-Tuñón, Ignacio Gutiérrez-Caballero, Cristina de Álava, Enrique Barbero, José Luis Schimenti, John de Rooij, Dirk G. Sánchez-Martín, Manuel Pendás, Alberto M. J Cell Biol Research Articles Cohesin is a conserved multisubunit protein complex that participates in chromosome segregation, DNA damage repair, chromatin regulation, and synaptonemal complex (SC) formation. Yeast, but not mice, depleted of the cohesin subunit Rec8 are defective in the formation of the axial elements (AEs) of the SC, suggesting that, in mammals, this function is not conserved. In this paper, we show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51- but not DMC1-mediated double-strand break repair, were not able to assemble their AEs, and arrested as early as the leptotene stage of prophase I, demonstrating that cohesin plays an essential role in AE assembly that is conserved from yeast to mammals. The Rockefeller University Press 2012-06-25 /pmc/articles/PMC3384418/ /pubmed/22711701 http://dx.doi.org/10.1083/jcb.201201100 Text en © 2012 Llano et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Llano, Elena
Herrán, Yurema
García-Tuñón, Ignacio
Gutiérrez-Caballero, Cristina
de Álava, Enrique
Barbero, José Luis
Schimenti, John
de Rooij, Dirk G.
Sánchez-Martín, Manuel
Pendás, Alberto M.
Meiotic cohesin complexes are essential for the formation of the axial element in mice
title Meiotic cohesin complexes are essential for the formation of the axial element in mice
title_full Meiotic cohesin complexes are essential for the formation of the axial element in mice
title_fullStr Meiotic cohesin complexes are essential for the formation of the axial element in mice
title_full_unstemmed Meiotic cohesin complexes are essential for the formation of the axial element in mice
title_short Meiotic cohesin complexes are essential for the formation of the axial element in mice
title_sort meiotic cohesin complexes are essential for the formation of the axial element in mice
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3384418/
https://www.ncbi.nlm.nih.gov/pubmed/22711701
http://dx.doi.org/10.1083/jcb.201201100
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