Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction

Human UDP-glucose 6-dehydrogenase (hUGDH) catalyzes the biosynthetic oxidation of UDP-glucose into UDP-glucuronic acid. The catalytic reaction proceeds in two NAD(+)-dependent steps via covalent thiohemiacetal and thioester enzyme intermediates. Formation of the thiohemiacetal adduct occurs through...

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Detalles Bibliográficos
Autores principales: Eixelsberger, Thomas, Brecker, Lothar, Nidetzky, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387377/
https://www.ncbi.nlm.nih.gov/pubmed/22525098
http://dx.doi.org/10.1016/j.carres.2012.03.028

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387377/
https://www.ncbi.nlm.nih.gov/pubmed/22525098
http://dx.doi.org/10.1016/j.carres.2012.03.028

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