T7 RNA Polymerase Functions In Vitro without Clustering

Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using ‘pulldowns’ and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)<1 µM. C...

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Detalles Bibliográficos
Autores principales: Finan, Kieran, Torella, Joseph P., Kapanidis, Achillefs N., Cook, Peter R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388079/
https://www.ncbi.nlm.nih.gov/pubmed/22768341
http://dx.doi.org/10.1371/journal.pone.0040207

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388079/
https://www.ncbi.nlm.nih.gov/pubmed/22768341
http://dx.doi.org/10.1371/journal.pone.0040207

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