A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression

BACKGROUND: Translocated in liposarcoma-CCAAT/enhancer binding protein homologous protein (TLS–CHOP) (also known as FUS-DDIT3) chimeric oncoprotein is found in the majority of human myxoid liposarcoma (MLS), but its molecular function remains unclear. METHODS: We knockdowned TLS–CHOP expression in M...

Descripción completa

Detalles Bibliográficos
Autores principales: Oikawa, K, Tanaka, M, Itoh, S, Takanashi, M, Ozaki, T, Muragaki, Y, Kuroda, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388565/
https://www.ncbi.nlm.nih.gov/pubmed/22588557
http://dx.doi.org/10.1038/bjc.2012.199
_version_ 1782237204459814912
author Oikawa, K
Tanaka, M
Itoh, S
Takanashi, M
Ozaki, T
Muragaki, Y
Kuroda, M
author_facet Oikawa, K
Tanaka, M
Itoh, S
Takanashi, M
Ozaki, T
Muragaki, Y
Kuroda, M
author_sort Oikawa, K
collection PubMed
description BACKGROUND: Translocated in liposarcoma-CCAAT/enhancer binding protein homologous protein (TLS–CHOP) (also known as FUS-DDIT3) chimeric oncoprotein is found in the majority of human myxoid liposarcoma (MLS), but its molecular function remains unclear. METHODS: We knockdowned TLS–CHOP expression in MLS-derived cell lines by a specific small interfering RNA, and analysed the gene expression profiles with microarray. RESULTS: TLS-CHOP knockdown inhibited growth of MLS cells, and induced an anticancer cytokine, melanoma differentiation-associated gene 7 (MDA-7)/interleukin-24 (IL-24) expression. However, double knockdown of TLS–CHOP and MDA-7/IL-24 did not inhibit MLS cell growth. CONCLUSION: Repression of MDA-7/IL-24 expression by TLS–CHOP is required for MLS tumour growth, and TLS–CHOP may become a promising therapeutic target for MLS treatment.
format Online
Article
Text
id pubmed-3388565
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-33885652013-06-05 A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression Oikawa, K Tanaka, M Itoh, S Takanashi, M Ozaki, T Muragaki, Y Kuroda, M Br J Cancer Short Communication BACKGROUND: Translocated in liposarcoma-CCAAT/enhancer binding protein homologous protein (TLS–CHOP) (also known as FUS-DDIT3) chimeric oncoprotein is found in the majority of human myxoid liposarcoma (MLS), but its molecular function remains unclear. METHODS: We knockdowned TLS–CHOP expression in MLS-derived cell lines by a specific small interfering RNA, and analysed the gene expression profiles with microarray. RESULTS: TLS-CHOP knockdown inhibited growth of MLS cells, and induced an anticancer cytokine, melanoma differentiation-associated gene 7 (MDA-7)/interleukin-24 (IL-24) expression. However, double knockdown of TLS–CHOP and MDA-7/IL-24 did not inhibit MLS cell growth. CONCLUSION: Repression of MDA-7/IL-24 expression by TLS–CHOP is required for MLS tumour growth, and TLS–CHOP may become a promising therapeutic target for MLS treatment. Nature Publishing Group 2012-06-05 2012-05-15 /pmc/articles/PMC3388565/ /pubmed/22588557 http://dx.doi.org/10.1038/bjc.2012.199 Text en Copyright © 2012 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Short Communication
Oikawa, K
Tanaka, M
Itoh, S
Takanashi, M
Ozaki, T
Muragaki, Y
Kuroda, M
A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression
title A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression
title_full A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression
title_fullStr A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression
title_full_unstemmed A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression
title_short A novel oncogenic pathway by TLS–CHOP involving repression of MDA-7/IL-24 expression
title_sort novel oncogenic pathway by tls–chop involving repression of mda-7/il-24 expression
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388565/
https://www.ncbi.nlm.nih.gov/pubmed/22588557
http://dx.doi.org/10.1038/bjc.2012.199
work_keys_str_mv AT oikawak anoveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT tanakam anoveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT itohs anoveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT takanashim anoveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT ozakit anoveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT muragakiy anoveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT kurodam anoveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT oikawak noveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT tanakam noveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT itohs noveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT takanashim noveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT ozakit noveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT muragakiy noveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression
AT kurodam noveloncogenicpathwaybytlschopinvolvingrepressionofmda7il24expression

Ejemplares similares