Crystallization of SHARPIN using an automated two-dimensional grid screen for optimization

An N-terminal fragment of human SHARPIN was recombinantly expressed in Escherichia coli, purified and crystallized. Crystals suitable for X-ray diffraction were obtained by a one-step optimization of seed dilution and protein concentration using a two-dimensional grid screen. The crystals belonged t...

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Detalles Bibliográficos
Autores principales: Stieglitz, Benjamin, Rittinger, Katrin, Haire, Lesley F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2012
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388930/
https://www.ncbi.nlm.nih.gov/pubmed/22750873
http://dx.doi.org/10.1107/S1744309112022208

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388930/
https://www.ncbi.nlm.nih.gov/pubmed/22750873
http://dx.doi.org/10.1107/S1744309112022208

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