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Crystallization of SHARPIN using an automated two-dimensional grid screen for optimization

An N-terminal fragment of human SHARPIN was recombinantly expressed in Escherichia coli, purified and crystallized. Crystals suitable for X-ray diffraction were obtained by a one-step optimization of seed dilution and protein concentration using a two-dimensional grid screen. The crystals belonged t...

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Detalles Bibliográficos
Autores principales:	Stieglitz, Benjamin, Rittinger, Katrin, Haire, Lesley F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2012
Materias:	Crystallization Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388930/ https://www.ncbi.nlm.nih.gov/pubmed/22750873 http://dx.doi.org/10.1107/S1744309112022208

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