PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System

For the site-specific labeling and modification of RNA by genetic alphabet expansion, we developed a PCR and transcription system using two hydrophobic unnatural base pairs: 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) as a third pair for PCR amplification and Ds and...

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Detalles Bibliográficos
Autores principales: Kimoto, Michiko, Yamashige, Rie, Yokoyama, Shigeyuki, Hirao, Ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3390107/
https://www.ncbi.nlm.nih.gov/pubmed/22792445
http://dx.doi.org/10.1155/2012/230943

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3390107/
https://www.ncbi.nlm.nih.gov/pubmed/22792445
http://dx.doi.org/10.1155/2012/230943

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