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A low-cost affinity purification system using β-1,3-glucan recognition protein and curdlan beads

Silkworm β-1,3-glucan recognition protein (βGRP) tightly and specifically associates with β-1,3-glucan. We report here an affinity purification system named the ‘GRP system’, which uses the association between the β-1,3-glucan recognition domain of βGRP (GRP-tag), as an affinity tag, and curdlan bea...

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Detalles Bibliográficos
Autores principales: Horiuchi, Masataka, Takahasi, Kiyohiro, Kobashigawa, Yoshihiro, Ochiai, Masanori, Inagaki, Fuyuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3390167/
https://www.ncbi.nlm.nih.gov/pubmed/22706764
http://dx.doi.org/10.1093/protein/gzs028