Quality-Score Refinement of SSU rRNA Gene Pyrosequencing Differs Across Gene Region for Environmental Samples

Due to potential sequencing errors in pyrosequencing data, species richness and diversity indices of microbial systems can be miscalculated. The “traditional” sequence refinement method is not sufficient to account for overestimations (e.g., length, primer errors, ambiguous nucleotides). Recent in s...

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Detalles Bibliográficos
Autores principales: Bowen De León, Kara, Ramsay, Bradley D., Fields, Matthew W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391548/
https://www.ncbi.nlm.nih.gov/pubmed/22476815
http://dx.doi.org/10.1007/s00248-012-0043-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3391548/
https://www.ncbi.nlm.nih.gov/pubmed/22476815
http://dx.doi.org/10.1007/s00248-012-0043-9

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