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Method optimization for proteomic analysis of soybean leaf: Improvements in identification of new and low-abundance proteins

The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the exis...

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Detalles Bibliográficos
Autores principales: Mesquita, Rosilene Oliveira, de Almeida Soares, Eduardo, de Barros, Everaldo Gonçalves, Loureiro, Marcelo Ehlers
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392888/
https://www.ncbi.nlm.nih.gov/pubmed/22802721
http://dx.doi.org/10.1590/S1415-47572012000200017