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Method optimization for proteomic analysis of soybean leaf: Improvements in identification of new and low-abundance proteins
The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the exis...
Autores principales: | Mesquita, Rosilene Oliveira, de Almeida Soares, Eduardo, de Barros, Everaldo Gonçalves, Loureiro, Marcelo Ehlers |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392888/ https://www.ncbi.nlm.nih.gov/pubmed/22802721 http://dx.doi.org/10.1590/S1415-47572012000200017 |
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