Testing the Limits of 454 Pyrotag Sequencing: Reproducibility, Quantitative Assessment and Comparison to T-RFLP Fingerprinting of Aquifer Microbes

The characterization of microbial community structure via 16S rRNA gene profiling has been greatly advanced in recent years by the introduction of amplicon pyrosequencing. The possibility of barcoding gives the opportunity to massively screen multiple samples from environmental or clinical sources f...

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Detalles Bibliográficos
Autores principales: Pilloni, Giovanni, Granitsiotis, Michael S., Engel, Marion, Lueders, Tillmann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395703/
https://www.ncbi.nlm.nih.gov/pubmed/22808168
http://dx.doi.org/10.1371/journal.pone.0040467

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395703/
https://www.ncbi.nlm.nih.gov/pubmed/22808168
http://dx.doi.org/10.1371/journal.pone.0040467

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