A Reverse Time-Course Method for Transcriptional Chase Analyses of mRNA Half-Lives in Cultured Cells

Standard methods for assessing mRNA stabilities in intact cells are labor-intensive and can generate half-life (t(1/2)) measures that are both imprecise and inaccurate. We describe modifications to a conventional tetracycline-conditional transcriptional chase method for analyzing mRNA stability that...

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Detalles Bibliográficos
Autores principales: Abdulmalik, Osheiza, Lombardi, Alyssa A., Russell, J. Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396636/
https://www.ncbi.nlm.nih.gov/pubmed/22808270
http://dx.doi.org/10.1371/journal.pone.0040827

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396636/
https://www.ncbi.nlm.nih.gov/pubmed/22808270
http://dx.doi.org/10.1371/journal.pone.0040827

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