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Isolation and Characterization of Simple Sequence Repeats (SSR) Markers from the Moss Genus Orthotrichum Using a Small Throughput Pyrosequencing Machine

Here, we report the results of next-generation sequencing on the GS Junior system to identify a large number of microsatellites from the epiphytic moss Orthotrichum speciosum. Using a combination of a total (non-enrichment) genomic library and small-scale 454 pyrosequencing, we determined 5382 conti...

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Detalles Bibliográficos
Autores principales: Sawicki, Jakub, Kwaśniewski, Mirosław, Szczecińska, Monika, Chwiałkowska, Karolina, Milewicz, Monika, Plášek, Vítězslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397546/
https://www.ncbi.nlm.nih.gov/pubmed/22837714
http://dx.doi.org/10.3390/ijms13067586