Cargando…

Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling

The functional cycle of the Rac1 GTPase involves a large number of steps, including post-translational processing, cytosolic sequestration by RhoGDIs, translocation to specific subcellular localizations, activation by GDP/GTP exchange, inactivation by GTP hydrolysis, and re-formation of cytosolic Ra...

Descripción completa

Detalles Bibliográficos
Autores principales: Bustelo, Xosé R., Ojeda, Virginia, Barreira, María, Sauzeau, Vincent, Castro-Castro, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398920/
https://www.ncbi.nlm.nih.gov/pubmed/22714419
http://dx.doi.org/10.4161/sgtp.19111
_version_ 1782238342812794880
author Bustelo, Xosé R.
Ojeda, Virginia
Barreira, María
Sauzeau, Vincent
Castro-Castro, Antonio
author_facet Bustelo, Xosé R.
Ojeda, Virginia
Barreira, María
Sauzeau, Vincent
Castro-Castro, Antonio
author_sort Bustelo, Xosé R.
collection PubMed
description The functional cycle of the Rac1 GTPase involves a large number of steps, including post-translational processing, cytosolic sequestration by RhoGDIs, translocation to specific subcellular localizations, activation by GDP/GTP exchange, inactivation by GTP hydrolysis, and re-formation of cytosolic Rac1/RhoGDI inhibitory complexes. Here, we summarize the current knowledge about the regulation of those steps. In addition, we discuss a recently described, cytoskeletal-dependent feed-back loop that favors the efficient translocation and activation of Rac subfamily proteins during cell signaling. This route is mediated by a heteromolecular protein complex composed of the cytoskeletal protein coronin1A, the Dbl family member ArhGEF7, the serine/threonine kinase Pak1, and the Rac1/RhoGDI dimer. This route promotes the translocation of Rac1/RhoGDI to F-actin-rich juxtamembrane areas, the Pak1-dependent release of Rac1 from the Rac1/RhoGDI complex, and Rac1 activation. This pathway is important for optimal Rac1 activation during the signaling of the EGF receptor, integrins, and the antigenic T-cell receptor.
format Online
Article
Text
id pubmed-3398920
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Landes Bioscience
record_format MEDLINE/PubMed
spelling pubmed-33989202012-07-18 Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling Bustelo, Xosé R. Ojeda, Virginia Barreira, María Sauzeau, Vincent Castro-Castro, Antonio Small GTPases Commentary The functional cycle of the Rac1 GTPase involves a large number of steps, including post-translational processing, cytosolic sequestration by RhoGDIs, translocation to specific subcellular localizations, activation by GDP/GTP exchange, inactivation by GTP hydrolysis, and re-formation of cytosolic Rac1/RhoGDI inhibitory complexes. Here, we summarize the current knowledge about the regulation of those steps. In addition, we discuss a recently described, cytoskeletal-dependent feed-back loop that favors the efficient translocation and activation of Rac subfamily proteins during cell signaling. This route is mediated by a heteromolecular protein complex composed of the cytoskeletal protein coronin1A, the Dbl family member ArhGEF7, the serine/threonine kinase Pak1, and the Rac1/RhoGDI dimer. This route promotes the translocation of Rac1/RhoGDI to F-actin-rich juxtamembrane areas, the Pak1-dependent release of Rac1 from the Rac1/RhoGDI complex, and Rac1 activation. This pathway is important for optimal Rac1 activation during the signaling of the EGF receptor, integrins, and the antigenic T-cell receptor. Landes Bioscience 2012-01-01 /pmc/articles/PMC3398920/ /pubmed/22714419 http://dx.doi.org/10.4161/sgtp.19111 Text en Copyright © 2012 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Commentary
Bustelo, Xosé R.
Ojeda, Virginia
Barreira, María
Sauzeau, Vincent
Castro-Castro, Antonio
Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling
title Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling
title_full Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling
title_fullStr Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling
title_full_unstemmed Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling
title_short Rac-ing to the plasma membrane: The long and complex work commute of Rac1 during cell signaling
title_sort rac-ing to the plasma membrane: the long and complex work commute of rac1 during cell signaling
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398920/
https://www.ncbi.nlm.nih.gov/pubmed/22714419
http://dx.doi.org/10.4161/sgtp.19111
work_keys_str_mv AT busteloxoser racingtotheplasmamembranethelongandcomplexworkcommuteofrac1duringcellsignaling
AT ojedavirginia racingtotheplasmamembranethelongandcomplexworkcommuteofrac1duringcellsignaling
AT barreiramaria racingtotheplasmamembranethelongandcomplexworkcommuteofrac1duringcellsignaling
AT sauzeauvincent racingtotheplasmamembranethelongandcomplexworkcommuteofrac1duringcellsignaling
AT castrocastroantonio racingtotheplasmamembranethelongandcomplexworkcommuteofrac1duringcellsignaling