Fast and Non-Toxic In Situ Hybridization without Blocking of Repetitive Sequences

Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However, in fluorescence in situ hybridization (FISH)...

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Detalles Bibliográficos
Autores principales: Matthiesen, Steen H., Hansen, Charles M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3404051/
https://www.ncbi.nlm.nih.gov/pubmed/22911704
http://dx.doi.org/10.1371/journal.pone.0040675

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3404051/
https://www.ncbi.nlm.nih.gov/pubmed/22911704
http://dx.doi.org/10.1371/journal.pone.0040675

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