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A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase

The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed an in silico analysis of the specificity and...

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Detalles Bibliográficos
Autores principales: Gaby, John Christian, Buckley, Daniel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405036/
https://www.ncbi.nlm.nih.gov/pubmed/22848735
http://dx.doi.org/10.1371/journal.pone.0042149