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A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed an in silico analysis of the specificity and...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2012
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405036/ https://www.ncbi.nlm.nih.gov/pubmed/22848735 http://dx.doi.org/10.1371/journal.pone.0042149 |