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Soft fibrin gels promote selection and growth of tumourigenic cells

The identification of stem-cell-like cancer cells through conventional methods that depend on stem-cell markers is often unreliable. We developed a mechanical method of selecting tumourigenic cells by culturing single cancer cells in fibrin matrices of ~100 Pa in stiffness. When cultured within thes...

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Detalles Bibliográficos
Autores principales: Liu, Jing, Tan, Youhua, Zhang, Huafeng, Zhang, Yi, Xu, Pingwei, Chen, Junwei, Poh, Yeh-Chuin, Tang, Ke, Wang, Ning, Huang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405191/
https://www.ncbi.nlm.nih.gov/pubmed/22751180
http://dx.doi.org/10.1038/nmat3361
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author Liu, Jing
Tan, Youhua
Zhang, Huafeng
Zhang, Yi
Xu, Pingwei
Chen, Junwei
Poh, Yeh-Chuin
Tang, Ke
Wang, Ning
Huang, Bo
author_facet Liu, Jing
Tan, Youhua
Zhang, Huafeng
Zhang, Yi
Xu, Pingwei
Chen, Junwei
Poh, Yeh-Chuin
Tang, Ke
Wang, Ning
Huang, Bo
author_sort Liu, Jing
collection PubMed
description The identification of stem-cell-like cancer cells through conventional methods that depend on stem-cell markers is often unreliable. We developed a mechanical method of selecting tumourigenic cells by culturing single cancer cells in fibrin matrices of ~100 Pa in stiffness. When cultured within these gels, primary human cancer cells or single cancer cells from mouse or human cancer cell lines grew within a few days into individual round colonies that resembled embryonic stem-cell colonies. Subcutaneous or intravenous injection of 10 or 100 fibrin-cultured cells in syngeneic or severe-combined-immunodeficiency mice led to the formation of solid tumours at the site of injection or at the distant lung organ much more efficiently than control cancer cells selected using conventional surface marker methods or cultured on conventional rigid dishes or on soft gels. Remarkably, as few as 10 such cells were able to survive and form tumours in the lungs of wild-type non-syngeneic mice.
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spelling pubmed-34051912013-02-01 Soft fibrin gels promote selection and growth of tumourigenic cells Liu, Jing Tan, Youhua Zhang, Huafeng Zhang, Yi Xu, Pingwei Chen, Junwei Poh, Yeh-Chuin Tang, Ke Wang, Ning Huang, Bo Nat Mater Article The identification of stem-cell-like cancer cells through conventional methods that depend on stem-cell markers is often unreliable. We developed a mechanical method of selecting tumourigenic cells by culturing single cancer cells in fibrin matrices of ~100 Pa in stiffness. When cultured within these gels, primary human cancer cells or single cancer cells from mouse or human cancer cell lines grew within a few days into individual round colonies that resembled embryonic stem-cell colonies. Subcutaneous or intravenous injection of 10 or 100 fibrin-cultured cells in syngeneic or severe-combined-immunodeficiency mice led to the formation of solid tumours at the site of injection or at the distant lung organ much more efficiently than control cancer cells selected using conventional surface marker methods or cultured on conventional rigid dishes or on soft gels. Remarkably, as few as 10 such cells were able to survive and form tumours in the lungs of wild-type non-syngeneic mice. 2012-07-01 /pmc/articles/PMC3405191/ /pubmed/22751180 http://dx.doi.org/10.1038/nmat3361 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Liu, Jing
Tan, Youhua
Zhang, Huafeng
Zhang, Yi
Xu, Pingwei
Chen, Junwei
Poh, Yeh-Chuin
Tang, Ke
Wang, Ning
Huang, Bo
Soft fibrin gels promote selection and growth of tumourigenic cells
title Soft fibrin gels promote selection and growth of tumourigenic cells
title_full Soft fibrin gels promote selection and growth of tumourigenic cells
title_fullStr Soft fibrin gels promote selection and growth of tumourigenic cells
title_full_unstemmed Soft fibrin gels promote selection and growth of tumourigenic cells
title_short Soft fibrin gels promote selection and growth of tumourigenic cells
title_sort soft fibrin gels promote selection and growth of tumourigenic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405191/
https://www.ncbi.nlm.nih.gov/pubmed/22751180
http://dx.doi.org/10.1038/nmat3361
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