Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing

Segmental isotopic labeling is a powerful labeling tool to facilitate NMR studies of larger proteins by not only alleviating the signal overlap problem but also retaining features of uniform isotopic labeling. Although two approaches, expressed protein ligation (EPL) and protein trans-splicing (PTS)...

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Detalles Bibliográficos
Autores principales: Minato, Yuichi, Ueda, Takumi, Machiyama, Asako, Shimada, Ichio, Iwaï, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405243/
https://www.ncbi.nlm.nih.gov/pubmed/22740268
http://dx.doi.org/10.1007/s10858-012-9628-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405243/
https://www.ncbi.nlm.nih.gov/pubmed/22740268
http://dx.doi.org/10.1007/s10858-012-9628-3

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