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F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization

Levels of G1 cyclins fluctuate in response to environmental cues and couple mitotic signaling to cell cycle entry. The G1 cyclin Cln3 is a key regulator of cell size and cell cycle entry in budding yeast. Cln3 degradation is essential for proper cell cycle control; however, the mechanisms that contr...

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Autores principales: Landry, Benjamin D., Doyle, John P., Toczyski, David P., Benanti, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405998/
https://www.ncbi.nlm.nih.gov/pubmed/22844257
http://dx.doi.org/10.1371/journal.pgen.1002851
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author Landry, Benjamin D.
Doyle, John P.
Toczyski, David P.
Benanti, Jennifer A.
author_facet Landry, Benjamin D.
Doyle, John P.
Toczyski, David P.
Benanti, Jennifer A.
author_sort Landry, Benjamin D.
collection PubMed
description Levels of G1 cyclins fluctuate in response to environmental cues and couple mitotic signaling to cell cycle entry. The G1 cyclin Cln3 is a key regulator of cell size and cell cycle entry in budding yeast. Cln3 degradation is essential for proper cell cycle control; however, the mechanisms that control Cln3 degradation are largely unknown. Here we show that two SCF ubiquitin ligases, SCF(Cdc4) and SCF(Grr1), redundantly target Cln3 for degradation. While the F-box proteins (FBPs) Cdc4 and Grr1 were previously thought to target non-overlapping sets of substrates, we find that Cdc4 and Grr1 each bind to all 3 G1 cyclins in cell extracts, yet only Cln3 is redundantly targeted in vivo, due in part to its nuclear localization. The related cyclin Cln2 is cytoplasmic and exclusively targeted by Grr1. However, Cdc4 can interact with Cdk-phosphorylated Cln2 and target it for degradation when cytoplasmic Cdc4 localization is forced in vivo. These findings suggest that Cdc4 and Grr1 may share additional redundant targets and, consistent with this possibility, grr1Δ cdc4-1 cells demonstrate a CLN3-independent synergistic growth defect. Our findings demonstrate that structurally distinct FBPs are capable of interacting with some of the same substrates; however, in vivo specificity is achieved in part by subcellular localization. Additionally, the FBPs Cdc4 and Grr1 are partially redundant for proliferation and viability, likely sharing additional redundant substrates whose degradation is important for cell cycle progression.
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spelling pubmed-34059982012-07-27 F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization Landry, Benjamin D. Doyle, John P. Toczyski, David P. Benanti, Jennifer A. PLoS Genet Research Article Levels of G1 cyclins fluctuate in response to environmental cues and couple mitotic signaling to cell cycle entry. The G1 cyclin Cln3 is a key regulator of cell size and cell cycle entry in budding yeast. Cln3 degradation is essential for proper cell cycle control; however, the mechanisms that control Cln3 degradation are largely unknown. Here we show that two SCF ubiquitin ligases, SCF(Cdc4) and SCF(Grr1), redundantly target Cln3 for degradation. While the F-box proteins (FBPs) Cdc4 and Grr1 were previously thought to target non-overlapping sets of substrates, we find that Cdc4 and Grr1 each bind to all 3 G1 cyclins in cell extracts, yet only Cln3 is redundantly targeted in vivo, due in part to its nuclear localization. The related cyclin Cln2 is cytoplasmic and exclusively targeted by Grr1. However, Cdc4 can interact with Cdk-phosphorylated Cln2 and target it for degradation when cytoplasmic Cdc4 localization is forced in vivo. These findings suggest that Cdc4 and Grr1 may share additional redundant targets and, consistent with this possibility, grr1Δ cdc4-1 cells demonstrate a CLN3-independent synergistic growth defect. Our findings demonstrate that structurally distinct FBPs are capable of interacting with some of the same substrates; however, in vivo specificity is achieved in part by subcellular localization. Additionally, the FBPs Cdc4 and Grr1 are partially redundant for proliferation and viability, likely sharing additional redundant substrates whose degradation is important for cell cycle progression. Public Library of Science 2012-07-26 /pmc/articles/PMC3405998/ /pubmed/22844257 http://dx.doi.org/10.1371/journal.pgen.1002851 Text en Landry et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Landry, Benjamin D.
Doyle, John P.
Toczyski, David P.
Benanti, Jennifer A.
F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization
title F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization
title_full F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization
title_fullStr F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization
title_full_unstemmed F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization
title_short F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization
title_sort f-box protein specificity for g1 cyclins is dictated by subcellular localization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3405998/
https://www.ncbi.nlm.nih.gov/pubmed/22844257
http://dx.doi.org/10.1371/journal.pgen.1002851
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