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Fluorescent In Situ Folding Control for Rapid Optimization of Cell-Free Membrane Protein Synthesis

Cell-free synthesis is an open and powerful tool for high-yield protein production in small reaction volumes predestined for high-throughput structural and functional analysis. Membrane proteins require addition of detergents for solubilization, liposomes, or nanodiscs. Hence, the number of paramete...

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Detalles Bibliográficos
Autores principales: Müller-Lucks, Annika, Bock, Sinja, Wu, Binghua, Beitz, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407079/
https://www.ncbi.nlm.nih.gov/pubmed/22848743
http://dx.doi.org/10.1371/journal.pone.0042186