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Fluorescent In Situ Folding Control for Rapid Optimization of Cell-Free Membrane Protein Synthesis
Cell-free synthesis is an open and powerful tool for high-yield protein production in small reaction volumes predestined for high-throughput structural and functional analysis. Membrane proteins require addition of detergents for solubilization, liposomes, or nanodiscs. Hence, the number of paramete...
Autores principales: | Müller-Lucks, Annika, Bock, Sinja, Wu, Binghua, Beitz, Eric |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407079/ https://www.ncbi.nlm.nih.gov/pubmed/22848743 http://dx.doi.org/10.1371/journal.pone.0042186 |
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