Single-Cell Pulsed-Field Gel Electrophoresis to Detect the Early Stage of DNA Fragmentation in Human Sperm Nuclei

Single-cell pulsed-field gel electrophoresis (SCPFGE) with dual electrode pairs was developed to detect the early stage of DNA fragmentation in human sperm. The motile sperm were purified by the commonly used density-gradient centrifugation technique and subsequent swim-up. The sperm were embedded i...

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Detalles Bibliográficos
Autores principales: Kaneko, Satoru, Yoshida, Joji, Ishikawa, Hiromichi, Takamatsu, Kiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407096/
https://www.ncbi.nlm.nih.gov/pubmed/22848752
http://dx.doi.org/10.1371/journal.pone.0042257

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407096/
https://www.ncbi.nlm.nih.gov/pubmed/22848752
http://dx.doi.org/10.1371/journal.pone.0042257

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