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Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin

BACKGROUND: Matrix metalloproteinase-2 (MMP-2) is a key regulator in the migration of tumor cells. αvβ3 integrin has been reported to play a critical role in cell adhesion and regulate the migration of tumor cells by promoting MMP-2 activation. However, little is known about the effects of MMP-2 on...

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Detalles Bibliográficos
Autores principales: Jiao, Yang, Feng, Xue, Zhan, Yinpeng, Wang, Ruifei, Zheng, Sheng, Liu, Wenguang, Zeng, Xianlu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407216/
https://www.ncbi.nlm.nih.gov/pubmed/22848537
http://dx.doi.org/10.1371/journal.pone.0041591
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author Jiao, Yang
Feng, Xue
Zhan, Yinpeng
Wang, Ruifei
Zheng, Sheng
Liu, Wenguang
Zeng, Xianlu
author_facet Jiao, Yang
Feng, Xue
Zhan, Yinpeng
Wang, Ruifei
Zheng, Sheng
Liu, Wenguang
Zeng, Xianlu
author_sort Jiao, Yang
collection PubMed
description BACKGROUND: Matrix metalloproteinase-2 (MMP-2) is a key regulator in the migration of tumor cells. αvβ3 integrin has been reported to play a critical role in cell adhesion and regulate the migration of tumor cells by promoting MMP-2 activation. However, little is known about the effects of MMP-2 on αvβ3 integrin activity and αvβ3 integrin-mediated adhesion and migration of tumor cells. METHODOLOGY/PRINCIPAL FINDINGS: Human melanoma cells were seeded using an agarose drop model and/or subjected to in vitro analysis using immunofluorescence, adhesion, migration and invasion assays to investigate the relationship between active MMP-2 and αvβ3 integrin during the adhesion and migration of the tumor cells. We found that MMP-2 was localized at the leading edge of spreading cells before αvβ3 integrin. αvβ3 integrin-mediated adhesion and migration of the tumor cells were inhibited by a MMP-2 inhibitor. MMP-2 cleaved fibronectin into small fragments, which promoted the adhesion and migration of the tumor cells. CONCLUSION/SIGNIFICANCE: MMP-2 cleaves fibronectin into small fragments to enhance the adhesion and migration of human melanoma cells mediated by αvβ3 integrin. These results indicate that MMP-2 may guide the direction of the tumor cell migration.
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spelling pubmed-34072162012-07-30 Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin Jiao, Yang Feng, Xue Zhan, Yinpeng Wang, Ruifei Zheng, Sheng Liu, Wenguang Zeng, Xianlu PLoS One Research Article BACKGROUND: Matrix metalloproteinase-2 (MMP-2) is a key regulator in the migration of tumor cells. αvβ3 integrin has been reported to play a critical role in cell adhesion and regulate the migration of tumor cells by promoting MMP-2 activation. However, little is known about the effects of MMP-2 on αvβ3 integrin activity and αvβ3 integrin-mediated adhesion and migration of tumor cells. METHODOLOGY/PRINCIPAL FINDINGS: Human melanoma cells were seeded using an agarose drop model and/or subjected to in vitro analysis using immunofluorescence, adhesion, migration and invasion assays to investigate the relationship between active MMP-2 and αvβ3 integrin during the adhesion and migration of the tumor cells. We found that MMP-2 was localized at the leading edge of spreading cells before αvβ3 integrin. αvβ3 integrin-mediated adhesion and migration of the tumor cells were inhibited by a MMP-2 inhibitor. MMP-2 cleaved fibronectin into small fragments, which promoted the adhesion and migration of the tumor cells. CONCLUSION/SIGNIFICANCE: MMP-2 cleaves fibronectin into small fragments to enhance the adhesion and migration of human melanoma cells mediated by αvβ3 integrin. These results indicate that MMP-2 may guide the direction of the tumor cell migration. Public Library of Science 2012-07-27 /pmc/articles/PMC3407216/ /pubmed/22848537 http://dx.doi.org/10.1371/journal.pone.0041591 Text en © 2012 Jiao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jiao, Yang
Feng, Xue
Zhan, Yinpeng
Wang, Ruifei
Zheng, Sheng
Liu, Wenguang
Zeng, Xianlu
Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin
title Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin
title_full Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin
title_fullStr Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin
title_full_unstemmed Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin
title_short Matrix metalloproteinase-2 Promotes αvβ3 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin
title_sort matrix metalloproteinase-2 promotes αvβ3 integrin-mediated adhesion and migration of human melanoma cells by cleaving fibronectin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3407216/
https://www.ncbi.nlm.nih.gov/pubmed/22848537
http://dx.doi.org/10.1371/journal.pone.0041591
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