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Synthetic Spike-in Standards Improve Run-Specific Systematic Error Analysis for DNA and RNA Sequencing
While the importance of random sequencing errors decreases at higher DNA or RNA sequencing depths, systematic sequencing errors (SSEs) dominate at high sequencing depths and can be difficult to distinguish from biological variants. These SSEs can cause base quality scores to underestimate the probab...
Autores principales: | Zook, Justin M., Samarov, Daniel, McDaniel, Jennifer, Sen, Shurjo K., Salit, Marc |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409179/ https://www.ncbi.nlm.nih.gov/pubmed/22859977 http://dx.doi.org/10.1371/journal.pone.0041356 |
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