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A diverse array of gene products are effectors of the type I interferon antiviral response
The type I interferon (IFN) response protects cells from invading viral pathogens. The cellular factors that mediate this defense are the products of interferon-stimulated genes (ISGs). Although hundreds of ISGs have been identified since their discovery over 25 years ago(1,2,3), only few have been...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409588/ https://www.ncbi.nlm.nih.gov/pubmed/21478870 http://dx.doi.org/10.1038/nature09907 |
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author | Schoggins, John W. Wilson, Sam J. Panis, Maryline Murphy, Mary Y. Jones, Christopher T. Bieniasz, Paul Rice, Charles M. |
author_facet | Schoggins, John W. Wilson, Sam J. Panis, Maryline Murphy, Mary Y. Jones, Christopher T. Bieniasz, Paul Rice, Charles M. |
author_sort | Schoggins, John W. |
collection | PubMed |
description | The type I interferon (IFN) response protects cells from invading viral pathogens. The cellular factors that mediate this defense are the products of interferon-stimulated genes (ISGs). Although hundreds of ISGs have been identified since their discovery over 25 years ago(1,2,3), only few have been characterized with respect to antiviral activity. For most, little is known about their antiviral potential, their target specificity, and their mechanisms of action. Using an overexpression screening approach, we show that different viruses are targeted by unique sets of ISGs, with each viral species susceptible to multiple antiviral genes with a range of inhibitory activities. To conduct the screen, over 380 ISGs were tested for their ability to inhibit the replication of several important viruses including hepatitis C virus (HCV), yellow fever virus (YFV), West Nile virus (WNV), chikungunya virus (CHIKV), Venezuelan equine encephalitis virus (VEEV), and human immunodeficiency virus (HIV-1). Broadly acting effectors included IRF1, C6orf150, HPSE, RIG-I, MDA5, and IFITM3, while more targeted antiviral specificity was observed with DDX60, IFI44L, IFI6, IFITM2, MAP3K14, MOV10, NAMPT, OASL, RTP4, TREX1, and UNC84B. Combined expression of two-ISG pairs showed additive antiviral effects similar to moderate IFN doses. Mechanistic studies revealed a common theme of translational inhibition for numerous effectors. Several ISGs, including ADAR, FAM46C, LY6E, and MCOLN2, enhanced replication of certain viruses, highlighting another layer of complexity in the highly pleiotropic IFN system. |
format | Online Article Text |
id | pubmed-3409588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
record_format | MEDLINE/PubMed |
spelling | pubmed-34095882012-08-01 A diverse array of gene products are effectors of the type I interferon antiviral response Schoggins, John W. Wilson, Sam J. Panis, Maryline Murphy, Mary Y. Jones, Christopher T. Bieniasz, Paul Rice, Charles M. Nature Article The type I interferon (IFN) response protects cells from invading viral pathogens. The cellular factors that mediate this defense are the products of interferon-stimulated genes (ISGs). Although hundreds of ISGs have been identified since their discovery over 25 years ago(1,2,3), only few have been characterized with respect to antiviral activity. For most, little is known about their antiviral potential, their target specificity, and their mechanisms of action. Using an overexpression screening approach, we show that different viruses are targeted by unique sets of ISGs, with each viral species susceptible to multiple antiviral genes with a range of inhibitory activities. To conduct the screen, over 380 ISGs were tested for their ability to inhibit the replication of several important viruses including hepatitis C virus (HCV), yellow fever virus (YFV), West Nile virus (WNV), chikungunya virus (CHIKV), Venezuelan equine encephalitis virus (VEEV), and human immunodeficiency virus (HIV-1). Broadly acting effectors included IRF1, C6orf150, HPSE, RIG-I, MDA5, and IFITM3, while more targeted antiviral specificity was observed with DDX60, IFI44L, IFI6, IFITM2, MAP3K14, MOV10, NAMPT, OASL, RTP4, TREX1, and UNC84B. Combined expression of two-ISG pairs showed additive antiviral effects similar to moderate IFN doses. Mechanistic studies revealed a common theme of translational inhibition for numerous effectors. Several ISGs, including ADAR, FAM46C, LY6E, and MCOLN2, enhanced replication of certain viruses, highlighting another layer of complexity in the highly pleiotropic IFN system. 2011-04-10 2011-04-28 /pmc/articles/PMC3409588/ /pubmed/21478870 http://dx.doi.org/10.1038/nature09907 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Schoggins, John W. Wilson, Sam J. Panis, Maryline Murphy, Mary Y. Jones, Christopher T. Bieniasz, Paul Rice, Charles M. A diverse array of gene products are effectors of the type I interferon antiviral response |
title | A diverse array of gene products are effectors of the type I interferon antiviral response |
title_full | A diverse array of gene products are effectors of the type I interferon antiviral response |
title_fullStr | A diverse array of gene products are effectors of the type I interferon antiviral response |
title_full_unstemmed | A diverse array of gene products are effectors of the type I interferon antiviral response |
title_short | A diverse array of gene products are effectors of the type I interferon antiviral response |
title_sort | diverse array of gene products are effectors of the type i interferon antiviral response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409588/ https://www.ncbi.nlm.nih.gov/pubmed/21478870 http://dx.doi.org/10.1038/nature09907 |
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