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Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy

Fluorophore excited state lifetime is a useful indicator of micro-environment in cellular optical molecular imaging. For quantitative sensing, precise lifetime determination is important, yet is often difficult to accomplish when using the experimental conditions favored by live cells. Here we repor...

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Detalles Bibliográficos
Autores principales: Chang, Ching-Wei, Mycek, Mary-Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3410727/
https://www.ncbi.nlm.nih.gov/pubmed/20588712
http://dx.doi.org/10.1364/OE.18.008688