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Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy
Fluorophore excited state lifetime is a useful indicator of micro-environment in cellular optical molecular imaging. For quantitative sensing, precise lifetime determination is important, yet is often difficult to accomplish when using the experimental conditions favored by live cells. Here we repor...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3410727/ https://www.ncbi.nlm.nih.gov/pubmed/20588712 http://dx.doi.org/10.1364/OE.18.008688 |