A Non-Destructive Culturing and Cell Sorting Method for Cardiomyocytes and Neurons Using a Double Alginate Layer

A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer...

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Detalles Bibliográficos
Autores principales: Terazono, Hideyuki, Kim, Hyonchol, Hayashi, Masahito, Hattori, Akihiro, Nomura, Fumimasa, Kaneko, Tomoyuki, Yasuda, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411729/
https://www.ncbi.nlm.nih.gov/pubmed/22870332
http://dx.doi.org/10.1371/journal.pone.0042485

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411729/
https://www.ncbi.nlm.nih.gov/pubmed/22870332
http://dx.doi.org/10.1371/journal.pone.0042485

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