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A Non-Destructive Culturing and Cell Sorting Method for Cardiomyocytes and Neurons Using a Double Alginate Layer
A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer...
Autores principales: | Terazono, Hideyuki, Kim, Hyonchol, Hayashi, Masahito, Hattori, Akihiro, Nomura, Fumimasa, Kaneko, Tomoyuki, Yasuda, Kenji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411729/ https://www.ncbi.nlm.nih.gov/pubmed/22870332 http://dx.doi.org/10.1371/journal.pone.0042485 |
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