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Biotin IgM Antibodies in Human Blood: A Previously Unknown Factor Eliciting False Results in Biotinylation-Based Immunoassays
Biotin is an essential vitamin that binds streptavidin or avidin with high affinity and specificity. As biotin is a small molecule that can be linked to proteins without affecting their biological activity, biotinylation is applied widely in biochemical assays. In our laboratory, IgM enzyme immuno a...
Autores principales: | Chen, Tingting, Hedman, Lea, Mattila, Petri S., Jartti, Laura, Jartti, Tuomas, Ruuskanen, Olli, Söderlund-Venermo, Maria, Hedman, Klaus |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411747/ https://www.ncbi.nlm.nih.gov/pubmed/22879954 http://dx.doi.org/10.1371/journal.pone.0042376 |
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