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HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells
We have demonstrated the anticancer effect of HRT in HCT116, human colon carcinoma cells. HRT inhibited cancer cell growth by causing cell cycle arrest at G(2)/M and inducing apoptosis as evidenced by DNA fragmentation assay. We found that HRT induces the activation of caspase-3, -8, and -9, whereas...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412102/ https://www.ncbi.nlm.nih.gov/pubmed/22899960 http://dx.doi.org/10.1155/2012/871893 |
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author | Yim, Nam-Hui Cho, Won-Kyung Lee, Ju Hye Jung, Young Pil Yang, Hye Jin Ma, Jin Yeul |
author_facet | Yim, Nam-Hui Cho, Won-Kyung Lee, Ju Hye Jung, Young Pil Yang, Hye Jin Ma, Jin Yeul |
author_sort | Yim, Nam-Hui |
collection | PubMed |
description | We have demonstrated the anticancer effect of HRT in HCT116, human colon carcinoma cells. HRT inhibited cancer cell growth by causing cell cycle arrest at G(2)/M and inducing apoptosis as evidenced by DNA fragmentation assay. We found that HRT induces the activation of caspase-3, -8, and -9, whereas it reduces the level of Bcl-2 protein and results in the cleavage of PARP. Further, HRT decreased the level of phosphorylation of Akt and its downstream signals such as mTOR and GSK-3β. These results indicate that HRT stimulates the apoptotic signaling pathway and represses the survival and proliferation of colon cancer cells via inhibiting Akt activity. Hence, our results suggest that HRT has a potential to be developed as a therapeutic agent against colon cancer cells. |
format | Online Article Text |
id | pubmed-3412102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34121022012-08-16 HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells Yim, Nam-Hui Cho, Won-Kyung Lee, Ju Hye Jung, Young Pil Yang, Hye Jin Ma, Jin Yeul Evid Based Complement Alternat Med Research Article We have demonstrated the anticancer effect of HRT in HCT116, human colon carcinoma cells. HRT inhibited cancer cell growth by causing cell cycle arrest at G(2)/M and inducing apoptosis as evidenced by DNA fragmentation assay. We found that HRT induces the activation of caspase-3, -8, and -9, whereas it reduces the level of Bcl-2 protein and results in the cleavage of PARP. Further, HRT decreased the level of phosphorylation of Akt and its downstream signals such as mTOR and GSK-3β. These results indicate that HRT stimulates the apoptotic signaling pathway and represses the survival and proliferation of colon cancer cells via inhibiting Akt activity. Hence, our results suggest that HRT has a potential to be developed as a therapeutic agent against colon cancer cells. Hindawi Publishing Corporation 2012 2012-07-26 /pmc/articles/PMC3412102/ /pubmed/22899960 http://dx.doi.org/10.1155/2012/871893 Text en Copyright © 2012 Nam-Hui Yim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yim, Nam-Hui Cho, Won-Kyung Lee, Ju Hye Jung, Young Pil Yang, Hye Jin Ma, Jin Yeul HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells |
title | HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells |
title_full | HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells |
title_fullStr | HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells |
title_full_unstemmed | HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells |
title_short | HRT, Herbal Formula, Induces G(2)/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells |
title_sort | hrt, herbal formula, induces g(2)/m cell cycle arrest and apoptosis via suppressing akt signaling pathway in human colon cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412102/ https://www.ncbi.nlm.nih.gov/pubmed/22899960 http://dx.doi.org/10.1155/2012/871893 |
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