A Nonradioactive Fluorimetric SPE-Based Ceramide Kinase Assay Using NBD-C(6)-Ceramide

Ceramide kinase (CERK) has been implicated in important cellular processes such as inflammation and apoptosis. Its activity is usually measured using radiolabeled ceramide or [γ-(32)P]-ATP, followed by extraction, thin-layer chromatography, and detection of the formed labeled ceramide-1-phosphate. T...

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Detalles Bibliográficos
Autores principales: Van Overloop, Helena, Van der Hoeven, Gerd, Van Veldhoven, Paul P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412103/
https://www.ncbi.nlm.nih.gov/pubmed/22900189
http://dx.doi.org/10.1155/2012/404513

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412103/
https://www.ncbi.nlm.nih.gov/pubmed/22900189
http://dx.doi.org/10.1155/2012/404513

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