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Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity

Substantial protection can be provided against the pre-erythrocytic stages of malaria by vaccination first with an adenoviral and then with an modified vaccinia virus Ankara (MVA) poxviral vector encoding the same ME.TRAP transgene. We investigated whether the two vaccine components adenovirus (Ad)...

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Autores principales: Reyes-Sandoval, Arturo, Rollier, Christine S, Milicic, Anita, Bauza, Karolis, Cottingham, Matthew G, Tang, Choon-Kit, Dicks, Matthew D, Wang, Dong, Longley, Rhea J, Wyllie, David H, Hill, Adrian VS
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412496/
https://www.ncbi.nlm.nih.gov/pubmed/22354374
http://dx.doi.org/10.1038/mt.2012.25
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author Reyes-Sandoval, Arturo
Rollier, Christine S
Milicic, Anita
Bauza, Karolis
Cottingham, Matthew G
Tang, Choon-Kit
Dicks, Matthew D
Wang, Dong
Longley, Rhea J
Wyllie, David H
Hill, Adrian VS
author_facet Reyes-Sandoval, Arturo
Rollier, Christine S
Milicic, Anita
Bauza, Karolis
Cottingham, Matthew G
Tang, Choon-Kit
Dicks, Matthew D
Wang, Dong
Longley, Rhea J
Wyllie, David H
Hill, Adrian VS
author_sort Reyes-Sandoval, Arturo
collection PubMed
description Substantial protection can be provided against the pre-erythrocytic stages of malaria by vaccination first with an adenoviral and then with an modified vaccinia virus Ankara (MVA) poxviral vector encoding the same ME.TRAP transgene. We investigated whether the two vaccine components adenovirus (Ad) and MVA could be coinjected as a mixture to enhance protection against malaria. A single-shot mixture at specific ratios of Ad and MVA (Ad+MVA) enhanced CD8(+) T cell-dependant protection of mice against challenge with Plasmodium berghei. Moreover, the degree of protection could be enhanced after homologous boosting with the same Ad+MVA mixture to levels comparable with classic heterologous Ad prime-MVA boost regimes. The mixture increased transgene-specific responses while decreasing the CD8(+) T cell antivector immunity compared to each vector used alone, particularly against the MVA backbone. Mixed vector immunization led to increased early circulating interferon-γ (IFN-γ) response levels and altered transcriptional microarray profiles. Furthermore, we found that sequential immunizations with the Ad+MVA mixture led to consistent boosting of the transgene-specific CD8(+) response for up to three mixture immunizations, whereas each vector used alone elicited progressively lower responses. Our findings offer the possibility of simplifying the deployment of viral vectors as a single mixture product rather than in heterologous prime-boost regimens.
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spelling pubmed-34124962012-08-06 Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity Reyes-Sandoval, Arturo Rollier, Christine S Milicic, Anita Bauza, Karolis Cottingham, Matthew G Tang, Choon-Kit Dicks, Matthew D Wang, Dong Longley, Rhea J Wyllie, David H Hill, Adrian VS Mol Ther Original Article Substantial protection can be provided against the pre-erythrocytic stages of malaria by vaccination first with an adenoviral and then with an modified vaccinia virus Ankara (MVA) poxviral vector encoding the same ME.TRAP transgene. We investigated whether the two vaccine components adenovirus (Ad) and MVA could be coinjected as a mixture to enhance protection against malaria. A single-shot mixture at specific ratios of Ad and MVA (Ad+MVA) enhanced CD8(+) T cell-dependant protection of mice against challenge with Plasmodium berghei. Moreover, the degree of protection could be enhanced after homologous boosting with the same Ad+MVA mixture to levels comparable with classic heterologous Ad prime-MVA boost regimes. The mixture increased transgene-specific responses while decreasing the CD8(+) T cell antivector immunity compared to each vector used alone, particularly against the MVA backbone. Mixed vector immunization led to increased early circulating interferon-γ (IFN-γ) response levels and altered transcriptional microarray profiles. Furthermore, we found that sequential immunizations with the Ad+MVA mixture led to consistent boosting of the transgene-specific CD8(+) response for up to three mixture immunizations, whereas each vector used alone elicited progressively lower responses. Our findings offer the possibility of simplifying the deployment of viral vectors as a single mixture product rather than in heterologous prime-boost regimens. Nature Publishing Group 2012-08 2012-02-21 /pmc/articles/PMC3412496/ /pubmed/22354374 http://dx.doi.org/10.1038/mt.2012.25 Text en Copyright © 2012 The American Society of Gene & Cell Therapy http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Reyes-Sandoval, Arturo
Rollier, Christine S
Milicic, Anita
Bauza, Karolis
Cottingham, Matthew G
Tang, Choon-Kit
Dicks, Matthew D
Wang, Dong
Longley, Rhea J
Wyllie, David H
Hill, Adrian VS
Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity
title Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity
title_full Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity
title_fullStr Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity
title_full_unstemmed Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity
title_short Mixed Vector Immunization With Recombinant Adenovirus and MVA Can Improve Vaccine Efficacy While Decreasing Antivector Immunity
title_sort mixed vector immunization with recombinant adenovirus and mva can improve vaccine efficacy while decreasing antivector immunity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412496/
https://www.ncbi.nlm.nih.gov/pubmed/22354374
http://dx.doi.org/10.1038/mt.2012.25
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