Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

BACKGROUND: Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allo...

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Detalles Bibliográficos
Autores principales: Ye, Jian, Coulouris, George, Zaretskaya, Irena, Cutcutache, Ioana, Rozen, Steve, Madden, Thomas L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412702/
https://www.ncbi.nlm.nih.gov/pubmed/22708584
http://dx.doi.org/10.1186/1471-2105-13-134

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412702/
https://www.ncbi.nlm.nih.gov/pubmed/22708584
http://dx.doi.org/10.1186/1471-2105-13-134

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