Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS

We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000–4,000 peptide-like features. Out of these, 730 p...

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Detalles Bibliográficos
Autores principales: Hölttä, Mikko, Zetterberg, Henrik, Mirgorodskaya, Ekaterina, Mattsson, Niklas, Blennow, Kaj, Gobom, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412831/
https://www.ncbi.nlm.nih.gov/pubmed/22880031
http://dx.doi.org/10.1371/journal.pone.0042555
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author Hölttä, Mikko
Zetterberg, Henrik
Mirgorodskaya, Ekaterina
Mattsson, Niklas
Blennow, Kaj
Gobom, Johan
author_facet Hölttä, Mikko
Zetterberg, Henrik
Mirgorodskaya, Ekaterina
Mattsson, Niklas
Blennow, Kaj
Gobom, Johan
author_sort Hölttä, Mikko
collection PubMed
description We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000–4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders.
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spelling pubmed-34128312012-08-09 Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS Hölttä, Mikko Zetterberg, Henrik Mirgorodskaya, Ekaterina Mattsson, Niklas Blennow, Kaj Gobom, Johan PLoS One Research Article We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000–4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders. Public Library of Science 2012-08-06 /pmc/articles/PMC3412831/ /pubmed/22880031 http://dx.doi.org/10.1371/journal.pone.0042555 Text en © 2012 Hölttä et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hölttä, Mikko
Zetterberg, Henrik
Mirgorodskaya, Ekaterina
Mattsson, Niklas
Blennow, Kaj
Gobom, Johan
Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_full Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_fullStr Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_full_unstemmed Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_short Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS
title_sort peptidome analysis of cerebrospinal fluid by lc-maldi ms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412831/
https://www.ncbi.nlm.nih.gov/pubmed/22880031
http://dx.doi.org/10.1371/journal.pone.0042555
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