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Characterization of a novel DNA polymerase activity assay enabling sensitive, quantitative and universal detection of viable microbes
During the past 50 years, in vitro measurement of DNA polymerase activity has become an essential molecular biology tool. Traditional methods used to measure DNA polymerase activity in vitro are undesirable due to the usage of radionucleotides. Fluorescence-based DNA polymerase assays have been deve...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413125/ https://www.ncbi.nlm.nih.gov/pubmed/22495933 http://dx.doi.org/10.1093/nar/gks316 |