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Characterization of a novel DNA polymerase activity assay enabling sensitive, quantitative and universal detection of viable microbes

During the past 50 years, in vitro measurement of DNA polymerase activity has become an essential molecular biology tool. Traditional methods used to measure DNA polymerase activity in vitro are undesirable due to the usage of radionucleotides. Fluorescence-based DNA polymerase assays have been deve...

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Detalles Bibliográficos
Autores principales: Zweitzig, Daniel R., Riccardello, Nichol M., Sodowich, Bruce I., O’Hara, S. Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413125/
https://www.ncbi.nlm.nih.gov/pubmed/22495933
http://dx.doi.org/10.1093/nar/gks316