Expression, Purification, and Mass Spectrometric Analysis of (15)N, (13)C-Labeled RGD-Hirudin, Expressed in Pichia pastoris , for NMR Studies

A novel recombinant hirudin, RGD-hirudin, inhibits the activity of thrombin and the aggregation of platelets. Here, we successfully expressed (15)N, (13)C-labeled RGD-hirudin in Pichia pastoris in a fermenter. The protein was subsequently purified to yield sufficient quantities for structural and fu...

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Detalles Bibliográficos
Autores principales: Huang, Yinong, Zhang, Yanling, Wu, Yi, Wang, Jue, Liu, Xingang, Dai, Linsen, Wang, Longsheng, Yu, Min, Mo, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413712/
https://www.ncbi.nlm.nih.gov/pubmed/22879918
http://dx.doi.org/10.1371/journal.pone.0042207

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413712/
https://www.ncbi.nlm.nih.gov/pubmed/22879918
http://dx.doi.org/10.1371/journal.pone.0042207

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