Fluorogenic DNA Sequencing in PDMS Microreactors

We have developed a multiplex sequencing-by-synthesis method combining terminal-phosphate labeled fluorogenic nucleotides (TPLFNs) and resealable microreactors. In the presence of phosphatase, the incorporation of a non-fluorescent TPLFN into a DNA primer by DNA polymerase results in a fluorophore....

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Detalles Bibliográficos
Autores principales: Sims, Peter A., Greenleaf, William J., Duan, Haifeng, Xie, X. Sunney
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414871/
https://www.ncbi.nlm.nih.gov/pubmed/21666670
http://dx.doi.org/10.1038/nmeth.1629

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3414871/
https://www.ncbi.nlm.nih.gov/pubmed/21666670
http://dx.doi.org/10.1038/nmeth.1629

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