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Metabolic trajectory of cellular differentiation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH

There is a lack of fast and high resolution methods to measure metabolic activity of single cells in their native environment. Here we develop a straightforward, non-invasive and sensitive method to measure metabolic phenotype of single cells in a live tissue. By using NADH as optical biomarker and...

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Detalles Bibliográficos
Autores principales: Stringari, Chiara, Edwards, Robert A., Pate, Kira T., Waterman, Marian L., Donovan, Peter J., Gratton, Enrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416911/
https://www.ncbi.nlm.nih.gov/pubmed/22891156
http://dx.doi.org/10.1038/srep00568