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Generation of Antibody-Producing Hybridomas Following One Single Immunization with a Targeted DNA Vaccine

The standard protocol for generating antibody (Ab)-producing hybridomas is based on fusion of plasmacytoma cells with Ab-producing B cells harvested from immunized mice. To increase the yield of hybridomas, it is important to use immunization protocols that induce a high frequency of B cells produci...

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Detalles Bibliográficos
Autores principales: Øynebråten, I, Løvås, T-O, Thompson, K, Bogen, B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417379/
https://www.ncbi.nlm.nih.gov/pubmed/21955209
http://dx.doi.org/10.1111/j.1365-3083.2011.02639.x