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Generation of Antibody-Producing Hybridomas Following One Single Immunization with a Targeted DNA Vaccine
The standard protocol for generating antibody (Ab)-producing hybridomas is based on fusion of plasmacytoma cells with Ab-producing B cells harvested from immunized mice. To increase the yield of hybridomas, it is important to use immunization protocols that induce a high frequency of B cells produci...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417379/ https://www.ncbi.nlm.nih.gov/pubmed/21955209 http://dx.doi.org/10.1111/j.1365-3083.2011.02639.x |