pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH

We report the development of a genetically encodable and ratiometic pH probe named “pHlash” that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation. The pHlash sensor–composed of a donor luciferase that is genetically fused to a Venus fluorophore–exhibits p...

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Detalles Bibliográficos
Autores principales: Zhang, Yunfei, Xie, Qiguang, Robertson, J. Brian, Johnson, Carl Hirschie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419163/
https://www.ncbi.nlm.nih.gov/pubmed/22905204
http://dx.doi.org/10.1371/journal.pone.0043072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419163/
https://www.ncbi.nlm.nih.gov/pubmed/22905204
http://dx.doi.org/10.1371/journal.pone.0043072

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