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pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH
We report the development of a genetically encodable and ratiometic pH probe named “pHlash” that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation. The pHlash sensor–composed of a donor luciferase that is genetically fused to a Venus fluorophore–exhibits p...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419163/ https://www.ncbi.nlm.nih.gov/pubmed/22905204 http://dx.doi.org/10.1371/journal.pone.0043072 |