Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition

The luciferase protein fragment complementation assay is a powerful tool for studying protein-protein interactions. Two inactive fragments of luciferase are genetically fused to interacting proteins, and when these two proteins interact, the luciferase fragments can reversibly associate and reconsti...

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Detalles Bibliográficos
Autores principales: Hatzios, Stavroula K., Ringgaard, Simon, Davis, Brigid M., Waldor, Matthew K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419657/
https://www.ncbi.nlm.nih.gov/pubmed/22905225
http://dx.doi.org/10.1371/journal.pone.0043175

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3419657/
https://www.ncbi.nlm.nih.gov/pubmed/22905225
http://dx.doi.org/10.1371/journal.pone.0043175

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