Structural and Functional Characterization of RecG Helicase under Dilute and Molecular Crowding Conditions

In an ATP-dependent reaction, the Escherichia coli RecG helicase unwinds DNA junctions in vitro. We present evidence of a unique protein conformational change in the RecG helicase from an α-helix to a β-strand upon an ATP binding under dilute conditions using circular dichroism (CD) spectroscopy. In...

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Detalles Bibliográficos
Autores principales: Saxena, Sarika, Nagatoishi, Satoru, Miyoshi, Daisuke, Sugimoto, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420092/
https://www.ncbi.nlm.nih.gov/pubmed/22919464
http://dx.doi.org/10.1155/2012/392039

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3420092/
https://www.ncbi.nlm.nih.gov/pubmed/22919464
http://dx.doi.org/10.1155/2012/392039

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